keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PPADS tetrasodiuma 是一种有效的 P2X 受体拮抗剂,也是是豚鼠气道平滑肌 Na/Ca²⁺ 交换逆模式的抑制剂,对谷氨酸/NMDA毒性有神经保护作用。PPADS tetrasodiuma 抑制 P2X1、P2X-2、P2X-3 和 P2X-5。
产品描述 | PPADS tetrasodiuma is a potent P2X receptor antagonist and inhibitor of the inverse mode of Na/Ca²⁺ exchange in guinea pig airway smooth muscle and is neuroprotective against glutamate/NMDA toxicity.PPADS tetrasodiuma inhibits P2X1, P2X-2, P2X-3, and P2X- 5. 5. |
靶点活性 | P2XRs:1-3 μM, P2X4 (human):30 μM |
体外活性 |
In a time- and concentration-dependent manner, PPADS tetrasodiuma (1-30 μM; 10-50 minutes) inhibits Na+/Ca2+ exchanger reverse mode (NCXREV)[2]. PPADS tetrasodiuma is effective at other native and recombinant P2XRs. Sensitivity to PPADS tetrasodiuma at human P2XRs depends on the subtype and is highest at the hP2X1, -2, -3, -5, and -7Rs with an IC50 of ~1-3 μM and ~30 μM for the hP2X4R[3]. |
体内活性 | In mesangial proliferative glomerulonephritis, PPADS tetrasodiuma (15-60 mg/100g body weight (BW); i.p.; every 12 hours for 8 days) inhibits the proliferation of glomerular mesangial cells (MC) without altering the proliferation of non-MC in vivo[4]. |
分子量 | 599.3 |
分子式 | C14H10N3Na4O12PS2 |
CAS No. | 192575-19-2 |
keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 40 mg/mL (66.74 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 1.6686 mL | 8.3431 mL | 16.6861 mL | 41.7153 mL |
5 mM | 0.3337 mL | 1.6686 mL | 3.3372 mL | 8.3431 mL | |
10 mM | 0.1669 mL | 0.8343 mL | 1.6686 mL | 4.1715 mL | |
20 mM | 0.0834 mL | 0.4172 mL | 0.8343 mL | 2.0858 mL | |
50 mM | 0.0334 mL | 0.1669 mL | 0.3337 mL | 0.8343 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PPADS tetrasodium 192575-19-2 Membrane transporter/Ion channel Neuroscience P2X Receptor Na+/Ca2+ Exchanger PPADS Inhibitor inhibitor inhibit